Journal: Development (Cambridge, England)
Article Title: Laminin α2 controls mouse and human stem cell behaviour during midbrain dopaminergic neuron development
doi: 10.1242/dev.172668
Figure Lengend Snippet: Concentration-dependent effects of lm211 are mediated by distinct receptor engagement. (A) Immunostaining of day 28 cultures treated with lm211 (4 μg ml−1) in combination with integrin blocking antibodies and a control condition of lm211 (1 μg ml−1). (B,C) Quantification of proliferation (B) and differentiation (C) following blocking of integrin α-subunit demonstrates that blocking both integrins α6 and α7 reverses the effect of the lm211-rich environment. (D) Images of day 28 cultures treated with laminin isoforms (1 μg ml−1) and dystroglycan blocking antibody or isotype control. (E,F) Quantification of proliferation (E) and differentiation (F) demonstrates that blockade of dystroglycan reverses the gain in mDA neurons seen in response to treatment with lm211 compared with lm111 without effecting proliferation. Unpaired two-tailed t-test; *P<0.0001, n=3. Scale bars: 50 μm in all images.
Article Snippet: Antibodies used were integrin α3 IA3 (R&D MAB1345-SP), integrin α6 GoH3 (R&D MAB13501), integrin α7 6A11 (LifeSpan Biosciences, LS-C179572), dystroglycan IIH6C4 (Millipore, 05-593), mouse-IgM (ThermoFisher, 02-6800), rat-IgG2a (ThermoFisher, 02-9688) and mouse-IgG1 (ThermoFisher, 02-6100).
Techniques: Concentration Assay, Immunostaining, Blocking Assay, Two Tailed Test